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          基因商城首頁 > 試劑 > 免疫學 > IP 免疫共沉淀(CHIP & MeDIP ) > MeDIP Kit

          SimpleDIP? Hydroxymethylated DNA IP (hMeDIP) Kit

          SimpleDIP? Hydroxymethylated DNA IP (hMeDIP) Kit

          品牌:CST 貨號:95176S 注冊及訂單:注冊及訂單 咨詢QQ 產品及技術:產品及技術 咨詢QQ

          商城價:

          ¥3695.00
          目錄價:¥4926.00

          特價促銷:

          ¥3695.00
          選擇包裝:
          庫存貨期:
          服  務:
          • 基因生物技術國際貿易(上海)有限公司 發貨并提供售后服務。
            本產品僅限科研用途。

          Shelf Life(month):12
          產地:USA
          存儲條件:2-8 & -20
          SimpleDIP?羥甲基化DNA免疫沉淀試劑盒;應用MeDIP ;儲存溫度4℃ and -20℃;
          The SimpleDIP? Hydroxymethylated DNA IP (hMeDIP) Kit provides enough reagents to perform up to 10 genomic DNA preparations and 10 IPs from mammalian cells and is optimized for 1 μg of genomic DNA per IP. The SimpleDIP? protocol can be performed in as little as two days and can easily be scaled up or down for use with more or less cells. Cells are first lysed and genomic DNA is extracted and sonicated into small fragments (200-500 bp). DNA IPs are performed using 5-Hydroxymethylcytosine (5-hmC) (HMC31) Mouse mAb and ChIP-Grade Protein G Magnetic Beads. After elution from the beads, the DNA is purified using DNA purification spin columns provided in the kit. The enrichment of particular DNA sequences can be analyzed by a variety of methods including standard PCR, quantitative real-time PCR, or next-generation sequencing. The SimpleDIP? 5-Hydroxymethylcytosine DNA IP Kit provides a highly validated 5-hmC monoclonal antibody to ensure specific and robust signal. The kit also includes DNA that contains exclusively 5-hydroxymethylcytosine, which can be spiked-in to the IPs as a control. Thus, spiked-in DNA will be immunoprecipitated with 5-Hydroxymethylcytosine (HMC1) Mouse mAb, but not with the Mouse (G3A1) mAb IgG1 Isotype Control (DIP Formulated). The relative enrichment can then be quantified using the SimpleDIP Hydroxymethyl Control Primers.
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          • 咨詢標題:[咨詢產品]CST / 95176S / SimpleDIP? Hydroxymethylated DNA IP (hMeDIP) Kit
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