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          基因商城首頁 > 試劑 > 細胞生物學 > 細胞分析 > 細胞增殖/毒性分析

          BrdU 細胞增殖分析試劑盒

          BrdU Cell Proliferation Assay Kit

          品牌:CST 貨號:6813S 注冊及訂單:注冊及訂單 咨詢QQ 產品及技術:產品及技術 咨詢QQ

          商城價:

          ¥5538.00
          目錄價:¥7384.00

          特價促銷:

          ¥5538.00
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          • 基因生物技術國際貿易(上海)有限公司 發貨并提供售后服務。
            本產品僅限科研用途。

          Shelf Life(month):6
          產地:USA
          存儲條件:-20
          BrdU Cell Proliferation Assay kit能夠檢測細胞增殖過程中摻入到細胞DNA中的BrdU,使用的抗體為抗BrDU抗體。當細胞培養于含BrDU的標記培養基中,該胸腺嘧啶類似物可代替胸腺嘧啶摻入到增殖細胞的新合成DNA中。吸除標記培養基后,用固定/變性液固定細胞同時使DNA變性。然后加入BrdU鼠源單抗檢測摻入的BrdU(DNA變性使得摻入的BrdU易于為抗體所識別)。Anti-mouse IgG, HRP-linked antibody用來識別聯胞檢測抗體。加入HRP底物(TMB)進行顯色。顯色過程中吸光度的強度和摻入細胞的BrdU的量成正比, BrdU是細胞增殖的直接證明。BrdU Cell Proliferation Assay kit能夠檢測細胞增殖過程中摻入到細胞DNA中的BrdU。BrDU標記的DNA變性后能夠為試劑盒中的BrdU Mouse mAb檢測到。BrdU Mouse mAb不與內源性DNA發生交叉反應。依細胞種類和孵育時間不同 ,每孔0.2-2x104個細胞即足夠大部分實驗。為了得到更佳實驗結果,我們推薦進行細胞數滴度實驗
          500 assays (96 well format). The BrdU Cell Proliferation Assay Kit detects 5-bromo-2’-deoxyuridine (BrdU) incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody. When cells are cultured with labeling medium that contains BrdU, this pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells. After removing labeling medium, cells are fixed and the DNA is denatured with our fixing/denaturing solution. Then a BrdU mouse mAb is added to detect the incorporated BrdU (The denaturing of DNA is necessary to improve the accessibility of the incorporated BrdU to the detection antibody). Anti-mouse IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate TMB is added to develop color. The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells, which is a direct indication of cell proliferation.
          BrdU Cell Proliferation Assay kit detects BrdU incorporation into cellular DNA during cell proliferation. The BrdU-labeled DNA has to be denatured to be detected by the BrdU Mouse mAb used in this kit. This BrdU Mouse mAb does not cross react with endogenous DNA. Depending on the cell type and the incubation time applied in the assay, 0.2-2x104 cells/well are sufficient for most experimental setups.
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